《植物生理学报》 2012, 48(6): 577-583

水稻叶绿素含量动态QTL分析

刘进¹, 王嘉宇^1,*, 姜树坤², 徐正进¹

1沈阳农业大学水稻研究所/农业部东北水稻生物学与遗传育种重点实验室, 沈阳110866; 2黑龙江省农业科学院耕作栽培研 究所, 哈尔滨150086

通信作者：王嘉宇；E-mail: ricewjy@126.com；Tel: 024-88487184

摘　要：

为剖析水稻不同生育时期叶绿素含量的变化动态及遗传机制, 以‘Sasanishiki’ (粳稻)、‘Habataki’ (籼稻)及其杂交衍生 的85个回交重组自交系(BILs)群体为材料, 对控制水稻叶片叶绿素含量的数量性状基因位点(QTL)变化动态进行了分析。 共检测到39个QTL, 包括26个非条件QTL和13个条件QTL, 分布在除第7和第11号染色体以外的10条染色体上, 平均每个时 期检测到3.25个非条件QTL。其中生育前期和生育中后期检测到的QTL位点较少, 仅为1~3个; 在生育中期(盛期)检测到的 QTL位点相对较多, 一般为4~5个。在生育期的中期和后期均能在第1和2号染色体上检测到控制叶绿素含量的QTL, 并且 这些QTL位点在1和2号染色体上呈现聚集现象。本研究同时发现了一些新的QTL位点, 这些QTL将有助于我们更全面地 了解叶绿素在不同发育时期的遗传基础。

关键词：水稻; 叶绿素含量; 数量性状位点; 动态分析

收稿：2012-01-30   修定：2012-04-23

资助：辽宁省博士科研启动基金项目(20091070)。

Detection and Analysis of Dynamic QTL of Leaf Chlorophyll Content in Rice (Oryza sativa L.)

LIU Jin¹, WANG Jia-Yu^1,*, JIANG Shu-Kun², XU Zheng-Jin¹

¹Rice Research Institute, Shenyang Agricultural University/Key Laboratory of Northeast Rice Biology and Breeding, Ministry of Agriculture, Shenyang 110866, China; ²Cultivation and Farming Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China

Corresponding author: WANG Jia-Yu; E-mail: ricewjy@126.com; Tel: 024-88487184

Abstract:

Dynamic changes of quantitative trait locus (QTL) controlling rice chlorophyll content were analyzed by using 85 backcross recombinant inbred lines (BILs) to explore the dynamic changes and genetic mechanism of rice chlorophyll content at different growth stages. The BILs were derived from a cross between ‘Sasanishiki’ (Oryza sativa var. japonica) and ‘Habataki’ (O. sativa var. indica). 39 QTLs including 26 unconditional and 13 conditional QTLs were detected on 10 chromosomes except the chromosome 7 and chromosome 11. QTLs were less both in early and late growth stages, in which only 1-3 QTLs were identified. Generally, 4-5 QTLs were detected in the mid-growth period. QTL clusters were both detected on the chromosomes 1 and 2 at mid-growth and late growth stages. Some new QTLs were also found in this study. All these QTLs information would help us to understand the genetic basis of chlorophyll at different stages in more detail.

Key words: Oryza sativa; chlorophyll content; quantitative trait locus; dynamic analysis